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2. Metz J, McGrath K, Bennett M, Hyland K, Bottiglieri T. Biochemical indices of vitamin B12 nutrition in pregnant patients with subnormal serum vitamin B12 levels. J Hematol 1995; 48: 251-5. Pardo J, Gindes L, Orvieto R. Cobalamin vitamin B12 ; metabolism during pregnancy. Int J Gynaecol Obstet 2004; 84: 77-8. Salawu L, Durosinmi MA. Erythrocyte rate and plasma viscosity in health and disease. Niger J Med 2001; 10: 11-3. Karim SA, Khurshid M, Rizvi JH, Jafarey SN, Rizwana I. Platelets and leucocyte counts in pregnancy. J Pak Med Assoc 1992; 42: 86-7 . 6. Kline JA, Williams GW, Hernandez-Nino J. D-dimer concentrations in normal pregnancy: new diagnostic thresholds are needed. Clin Chem 2005; 51: 825-9 Wickstrom K, Edelstam G, Lowbeer CH, Hansson LO, Siegbahn A. Reference intervals for plasma levels of fibronectin, von Willebrand factor, free protein S and antithrombin during third-trimester pregnancy. Scand J Clin Lab Invest 2004; 64: 31-40 and vicoprofen. Utes after the injection with appropriate corrections and standardisation for the patient's surface area 57-59 ; . When progression in any chronic kidney disease is evaluated development of ESDR is the ultimate endpoint. Since it takes several years to reach ESRD clinical trials in progressive kidney disease often requires other endpoints. The rate of decline in GFR has been approved as an endpoint by the Food and Drug Administration USA ; . The rate of decline in GFR was only calculated for patients completing follow-up 44; 46 ; or was calculated as the difference between first and last GFR determination in all participating patients 51 ; . 4.1.2 Urinary albumin excretion rate Due to large day to day variation in UAER 60 ; three 24-hour urinary collections were performed at each designated time point in all our studies. Indeed six 24-hour collections were used to confirm microalbuminuria at baseline in two studies 44; 46 ; . Timed urinary collections were used as it is widely accepted as the most accurate method for determination of UAER 61 ; . The urinary albumin concentration was determined by an immunoassay method 62 ; . Normoalbuminuria was defined as UAER 30 mg per 24 hour, microalbuminuria as UAER 30-300 mg per 24 hour, and macroalbuminuria as UAER 300 mg per 24 hour as defined at a consensus conference 63 ; . The fractional clearance of albumin was calculated by dividing the clearance of albumin calculated as UV P, where U is urine albumin concentration mg l ; , V is urine flow l 24 h ; , and P is plasma albumin concentration g l with the simultaneous measured GFR to correct albumin excretion for changes in plasma albumin concentration and in GFR 51 ; . 4.2 ENDOTHELIAL DYSFUNCTION Endothelial function was evaluated by determining the transcapillary escape rate of albumin TERalb ; in one study 51 ; and with measurement of the serum concentration of von Willebrand factor vWF ; after an overnight fast 48 ; . 4.2.1 Transcapillary escape rate TERalb ; TERalb is defined as the fraction of the intravascular mass of albumin that passes to the extravascular space per unit of time percent per hour ; . It is determined as the rate constant of the practically monoexponential decrease in plasma radioactivity over the first 60 minutes following injection of tracer albumin as calculated by the least squares method as described 64 ; . 4.2.2 Von Willebrand factor Von Willebrand factor is a high molecular weight glycoprotein synthesised mainly by the endothelial cells and acts as a non-specific marker of endothelial dysfunction 65; 66 ; . The plasma concentration of vWF was measured by microenzyme linked immunoadsorbent assay as described by Ingerslev 67 ; . A close agreement between plasma and serum levels of vWF has been described 68 ; . 4.3 LABORATORY ASSAYS 4.3.1 Glycaemic regulation Glycosylated haemoglobin A1c was determined by ion-exchange high-performance liquid chromatography HPLC ; Bio-Rad VARIANT, California, USA ; and the non-diabetic reference range in our laboratory was 4.1-6.4%. Blood glucose was measured by a glucose oxidase method. 4.3.2 Lipid profiles Venous blood samples were drawn after a 12 hour fast. Serum total cholesterol and serum high density lipoprotein HDL ; -cholesterol were measured by chromatography and serum triglycerides were measured by colorimetry. Serum low density lipoprotein LDL ; cholesterol was calculated by the Friedewald formula 69 ; in patients with a serum triglyceride concentration lower than 5 mmol l. 260.
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Triamterene is in the fda pregnancy category this means that it is not expected to be harmful to an unborn baby. Hydrochlorothiazide; triamterene is indicated for the treatment of hypertension or edema in patients who develop hypokalemia on hydrochlorothiazide alone.

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