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Atayde, V.D., Neira, I., Cortez, M., Ferreira, D., Herrera, F. and Yoshida, N. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de So Paulo, R. Botucatu, 862, So Paulo, SP, Brasil. e-mail : vdatayde ecb.epm We investigated the properties of metacyclic trypomastigotes of non virulent T. cruzi clone CL-14, as compared to the parental isolate CL. In contrast to the highly infective CL isolate, which produces high parasitemias in mice by intraperitoneal or oral administration, metacyclic forms of clone CL-14 failed to produce patent infection. In vitro, the number of clone CL-14 parasites that entered epithelial HeLa cells, after 1 h incubation, was ~4-fold lower than that of the CL isolate and, at 72 h post-infection, intracellular replication was not apparent whereas HeLa cells infected with CL isolate contained large number of parasites replicating as amastigotes. The stage-specific surface glycoprotein gp82 plays a central role in mucosal invasion of CL isolate metacyclic forms, leading to systemic infection upon oral challenge Neira et al., 2003, Inf. Immun. 71: 557-561 ; and is involved in host cell invasion in vitro Ruiz et al., 1998, Biochem. J. 330: 505-511 ; . Analysis by flow cytometry revealed that metacyclic forms of clone CL-14 express reduced levels of gp82 on the surface, although its overall expression, detectable by immunoblotting, is comparable to that of CL isolate, indicating that in clone CL-14 gp82 is mostly localized intracellularly. Otherwise, the surface profile of CL isolate and clone CL-14 was similar. Internally located clone CL-14 gp82, wich is not accessible to MAb 3F6, had its reactivity augmented by permeabilization of parasites with saponin. HeLa cell entry of clone CL-14 metacyclic forms increased upon treatment of parasites with neuraminidase, which removes sialic acid from the surface glycoprotein gp35 50, a mucin-like molecule previously shown to be engaged in cell invasion of poorly infective T. cruzi isolates Ruiz et al., 1998 ; . Activity of cystein proteinase cruzipain, reported to participate in host cell invasion and intracellular multiplication Meirelles et al., 1992, Biochem. Parasitol. 52: 175-184 ; , was similar in CL isolate and clone CL-14. Taken together, these data suggest that the lack of virulence of clone CL-14 is associated with the reduced expression of surface gp82 and the possible participation of gp35 50 in host cell invasion. Work supported by Fapesp, for instance, urispas.
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All the community women interviewed were questioned about STI HIV AIDS to assess their knowledge. The information is presented in Table 6.17. About 86 percent in Surya clinics and 78 percent of the respondents in Titli expressed that they had heard about the diseases can be contracted through sexual intercourse. However, only 57 percent in Surya and 29 percent in Titli areas had heard about HIV or AIDS. Further, only two-fifths in Surya and one-sixth in Titli mentioned that the use of a FP method could protect against STIs and HIV. Nearly 94 percent in Surya and all in Titli who had heard of FP methods that offer protection against STI HIV AIDS, knew that condom provides such protection.

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Table 2: Results of Androutsopoulos et al., 2000 ; on the LingSpam corpus. In the pre-processing column: 'bare' indicates no preprocessing. The column labelled 'No. of attrib.' indicates the number of word features which the authors retained as indicators of class. Results are shown at the bottom of the table from ST classification using a linear significance function and no normalisation; for the ST classifier, we performed no pre-processing and no feature selection. Pre-processing NB a ; bare b ; stop-list c ; lemmatizer d ; lemmatizer + stop-list a ; bare b ; stop-list c ; lemmatizer d ; lemmatizer + stop-list a ; bare b ; stop-list c ; lemmatizer d ; lemmatizer + stop-list bare bare No. of attrib. 50 100 N A N 1.0 SR % ; 81.10 82.35 SP % ; 96.85 97.13 99.02 Table 3: Results of in-house nave Bayes on the LS-FULL data set, with stop-words removed and all remaining words lemmatized. The number of attributes was unlimited, but, for the LS-FULL data set, in practice the spam vocabulary was approximately 12, 000, and the ham vocabulary approximately 56, 000, with 7, 000 words appearing in both classes. Pre-processing NB * lemmatizer + stop-list lemmatizer + stop-list No. of attrib. unlimited unlimited th 1.0 0.94 SR % ; 99.16 89.58 SP % ; 97.14 100.00. F9999 FINAL OBSERVATIONS STATE LICENSURE VIOLATIONS: STATE LICENSURE VIOLATIONS 300.1210b ; 1 ; 300, 1210b ; 2 ; 300.1630c ; 300.1630e ; Section 300.1210 General Requirements for Nursing and Personal Care b ; General nursing care shall include at a minimum the following and shall be practiced on a 24-hour, seven day a week basis: 1 ; Medications including oral, rectal, hypodermic, intravenous and intramuscular shall be properly administered. 2 ; All treatments and procedures shall be administered as ordered by the physician. Section 300.1630 Administration of Medication c ; Medications prescribed for one resident shall not be administered to another resident. e ; Medication errors and drug reactions shall be immediately reported to the resident's physician, licensed prescriber if other than a physician, the consulting pharmacist and the dispensing pharmacist if the consulting pharmacist and dispensing pharmacist are not associated with the same pharmacy ; . An entry shall be made in the resident's clinical record, and the error or reaction shall also be described in an incident report.
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